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Question 1 of 30

Histochemical staining used for diagnosing Hirschsprung's disease is

Rationale:
Acetyl choline esterase is the histochemical stain used for diagnosing Hirschsprung's disease. This stain highlights increased acetylcholinesterase activity in hypertrophic nerve fibers of aganglionic bowel segments, aiding identification of the disease. It provides a reliable marker for absence of ganglion cells, distinguishing affected tissue from normal, thus serving as a crucial diagnostic tool in Hirschsprung's disease evaluation. B: Dopamine does not relate to histochemical diagnosis of Hirschsprung's disease and lacks specificity for identifying aganglionic segments or nerve fiber hypertrophy within affected bowel tissue, making it unsuitable for this purpose. C: Glutamate is primarily involved in neurotransmission and lacks diagnostic relevance in Hirschsprung's disease, offering no distinctive staining pattern for aganglionosis or nerve fiber changes within the colon. D: Chromogranin and synaptophysin mark neuroendocrine cells but do not specifically detect the absence of ganglion cells or increased acetylcholinesterase activity, limiting their utility in diagnosing Hirschsprung's disease.